Dehyrated and Broth Media including NEW dehydrated media pads

M-Tech supplies a variety of dehydrated media pads, as well as a range of agars and broth media.

Dehydrated nutrient pad sets

Nutrient pad sets (NPS) are sterile, dehydrated culture media, ready for immediate use after addition of water. Shelf life of the sealed original package at room temperature is at least one year. Nutrient pads (NPs) are obligatorily used in combination use with membrane filtration technique.

The pads are made of biologicaly inert cellulose cardboard that does not bind the nutrient medium chemically as compared to conventional agar culture media.

Also available for M-Tech: CHROMagar ECC Pads.

The following range of NPS from Dr.Möller and Schmelz are available from M-Tech:

Medium Art.No	Description and notes	Incubation time and temp
Actinomycete-NPS 1005	Medium recomended for isolating and propogating Actinomycetes from soil, water, food and other samples, acc to Olson, mod. Note: Actinomycetes form gristly colonies, similar to small colonies of yeasts or moulds, which are firmly fixed to the memrane. With 0.45µm white, gridded membrane filter.	2-5d 30ºC
Azide-NPS 1010	Medium selective for fecal streptococci in water, food and other samples. Note: Streptococci form small dark-red colonies. With 0.45µm white, gridded membrane filter.	24-48h 37ºC
Beer-NPS 1020	Medium selective for beer-spoiling microbes (e.g. Lactobacillus, Pediococcus and Zymomonas) Note: Yeast growth is inhibited by Actidione (cycloheximide). With 0.45µm green, gridded membrane filter.	2-5d 30ºC (anaerobic)
Caso-NPS 1030	For colony count determinations and detection of demanding or sublethaly damaged microorganisms in pharaceuticals, cosmetics and other products. (USP) With 0.45µm green, gridded membrane filter.	48-72h 30ºC
Cetrimide-NPS 1040	Medium selective for Pseudomonas aeruginosa in water, pharmaceuticals, cosmetics and other samples. (USP, DIN) Note: Ps.aeruginosa form blue colonies with blue halos. With 0.45µm white, gridded membrane filter.	48h 37ºC
Chapman-NPS 10500	Medium selective for pathogenic staphylococci in food, phamaceuticals, cosmetics and clinical samples, (USP, APHA) Note: Staphylococcus aureus form yellow colonies with yellow halos. With 0.45µm white, gridded membrane filter.	48h 37ºC
China Blue-Lactose-NPS 1060	For colony count determinations and for the determination of acidic and non-acidic producers from milk and other dairy products. Note: Acid produced from lactose is indicated by blue colonies. With 0.45µm white, gridded membrane filter.	24-48h 30ºC
ECD-NPS (with indolkit) 1080	For selective and quantitative direct enumeration of E.coli within 24h in water, food and other samples. Note: Preincubation on Ressuscitation-NPS allows detection of sublethally damaged E.coli which are not found with different protocols. After incubation on ECD-NPS, the membrane is placed on a filter soaked with indole reagent. Within 1-2 minutes red-dish colonies with a reddish halo indicate indole formation. With 0.45µm white, gridded membrane filter.	10-20h 44ºC
ECD-MUG-NPS 1081	For the selective and quantitative direct enumeration of E.coli within 24h in water, food and other samples. Note: After incubation on membrane filter, collected colonies are lit up with UV light (366nm). Greenish flourescent colonies indicate MUG-dissociation. After this put some drops of Indol reagent in the lid of the petri dish and put the membrane filter on it. Within 1-2 minutes reddish colonies with a reddish halo indicate Indol formation. White a 0.45µm white, gridded membrane filter.	10-20h 44ºC
Endo-NPS 1090	Selective medium for E.coli and coliform bacteria in water, food and other samples. (DEV, APHA) Note: E.coli develop dark red colonies with a greenish metalic surface sheen. With 0.45µm white, gridded membrane filter.	24h, 37ºC or 42-44ºC
Glucose Typtone-NPS 1070	For colony count determinations of mesophillic bactera and for the detection of thermophillic spore formers in sugar and food. Note: "Flat-sour" colonies (B.coagulans) are greenish-yellow with a yellow halo. With 0.45µm white, gridded membrane filter.	2-3d, 30ºC or 1-2d,55ºC
M-FC-NPS 1100	Selective medium for E.coli and fecal coliforms in water, food and other samples. Note: Normally E.coli develop blue colonies within 16h. With 0.45µm white, gridded membrane filter.	16-24h 44ºC
MacConkey-NPS 1105	Medium for differential diagnosis and detection of E.coli, coliform bacteria and lactose-negative salmonelles and sgigelles in water, food and clinical samples. Note: E.coli form red colonies with a red halo, colonies of coliform bacteria are $plusmn; pink, while salmonelles and shigelles form colourless colonies. Accompanying gram-positive bacteria will be inhibited to a large extent. With 0.45µm white, gridded membrane filter.	24h 37ºC
MRS-NPS 1110	Selective medium for lactobacilli in food and other samples. Note: Growth of yeasts and moulds is inhibited by Actidione (cycloheximide). With 0.45µm white, gridded membrane filter.	3-5d 30ºC (anaerobic)
Orange Serum-NPS 1120	Selective medium for acidophillic and acidotolerant microbes in beverages and food. (APHA) Note: Anaerobic incubation also mediates growth of the more demanding lactobacilli. With 0.45µm green, gridded membrane filter.	2-3d 30ºC
Osmophil-NPS 1130	Selective medium for osmophillic and osmotolerant moulds in sugar, sweets and sugarised food. (ICUMSA) With 1.2µm green, gridded membrane filter.	5-7d 30ºC
Plate Count-NPS 1140	For colony count determinations of water, milk, food and other samples. (DLG, APHA) With 0.45µm green, gridded membrane filter.	48-72h 30ºC
Resuscitation-NPS 1150	Medium for optimal detection of sublethaly damaged enteric bacteria, pseudomonas and staphylococci by preincubation followed by main incubation with selective media.	2-4h, 37ºC or 14-16h, 20ºC
Sabouraud-NPS 1160	For detection of yeasts and moulds in phamaceuticals, cosmetics, packing material, for isolation of dermatophytes and for pure cultures. (USP) With 1.2µm green, gridded membrane filter	2-5d 25-30ºC
Schaedler-NPS 1170	For colony count determinations and the detection of demanding aerobic and anaerobic microorganisms in clinical and pharmaceutical material and cosmetics. With 0.45µm green, gridded membrane filter.	2-3d 30ºC
Schaufus-Pottinger-NPS 1180	Selective medium for yeasts and moulds in beverages and sugar. Note: Sugar fermenters develop yellowish colonies, non-acid formers are blue-green. (ICUMSA) With 1.2µm green gridded membrane filter.	2-3d 30ºC
Standard-NPS 1190	For colony count determinations of water and waste water, for pure cultures and as a base medium for further additions to the demineralised water (serum, antibiotics, etc) when wetting the pad. (DEV) With 0.45µm green, gridded membrane filter.	48-78h, 20ºC or 48h, 30ºC
Standard TTC-NPS 1200	see above, modified by the addition of triphenyltetrazoliumchloride (TTC) Note: Routine tests are easy to evaluate with the naked eye because all bacteria develop pink colonies (formazan formation from TTC) With 0.45µm green, gridded membrane filter.	see above
Teepol-NPS 1210	Selective medium for the detection of E.coli and fecal coliforms in water, food and other samples. Note: By preincubation (6h at 25ºC), sublethaly damaged bacteria can also be traced. E.coli grow as yellow colonies. With 0.45µm white, gridded membrane filter.	18-24h, 44ºC
Tergitol TTC-NPS 1220	Selective medium for the detection of E.coli and coliform bacteria in water, food and other samples. Note: After 12-16h (first inspection) E.coli develop small yellowish colonies which later change to orange and develop a yellow halo. With 0.45µm white, gridded membrane filter.	12-24h, 37ºC
Weman-NPS 1240	Selective medium for slime forming bacteria in sugar, beverages and food. (ICUMSA) Note: Slime formers e.g.Leuconostock, develop colourless water drop like colonies. With 0.45µm green, gridded membrane filter.	2-3d, 30ºC
Wine-NPS 1230	Selective medium for the detection of wine spoiling microbes (Leuconostock, Lactobacillus and Pediococcus). Improved tomato-juice medium. Note: Yeast growth is inhibited by Actidione (cycloheximide). Under aerobic conditions Acetobacter can also be detected. With 0.45µm greem, gridded membrane filter.	2-5d, 30ºC (anaerobic)
Bismuth Sulfite-NPS 1250	Selective medium for detection of Salmonella in water, food and clinical samples. Note: Salmonella for dark brown to black colonies surrounded by a black zone with a pale halo ("fish-eye"). (USP, APHA)	24-48h, 37ºC
Wort-NPS 1260	Selective medium for the detection of yeasts and moulds in beverages, food and other samples. With 1.2µm green, gridded membrane filter or 0.6µm black, gridded membrane filter.	2-5d, 25ºC

Note: Growth and positive results with the selective media should not lead rash colnclusions. For safe diagnosis further tests are necessary (e.g."IMVIC-Tests"). National regulations concerning infectious materials must be observed closely. Further information and application procedures are available on request and free of charge